Differences in Antibacterial Activity of Petroleum Ether and Ethanol Extracts of Bangle Rhizome (Zingiber Montanun (J. Koenig)) Against Staphylococcus aureus and Escherichia coli Bacteria
DOI:
https://doi.org/10.35473/ijpnp.v9i1.4942Keywords:
Antibacterial, PE Extract, Ethanol, S.Aureus, E.ColiAbstract
Disease-causing bacteria are divided into two groups: Gram-positive and Gram-negative. These bacteria have different cell wall characteristics, which affect treatment options. The aim of this study was to determine the differences in antibacterial activity of petroleum ether and 96% ethanol extracts against S. aureus and E. coli. The extraction method used was reflux. Antibacterial activity was measured using the well diffusion method with Mannitol Salt Agar and Eosin Methylene Blue Agar media. The results showed that PE extract 5.25% and ethanol 11.09%. The inhibition zone (cm) of antibacterial activity of PE extract on S. aureus bacteria at concentrations of 1%, 5%, and 10% was 1.38±0.155; 1.58±0.138; 1.72±0.129, respectively, while E. coli bacteria were 1.16±0.123; 1.37±0.212; 1.52±0.238. The inhibition zone of ethanol extract on S. aureus bacteria at concentrations of 1%, 5%, and 10% were 0; 1.39±0.136; 1.63±0.150, respectively, while E. coli bacteria were 1.30±0.191; 1.51±0.145; 1.54±0.159. Based on SPSS analysis, non-parametric test showed that the activity of PE and 96% ethanol extracts on S. aureus bacteria was significantly different from E. coli bacteria with a sig. value <0.05 and between PE and ethanol extracts provided significantly different activity against one type of bacteria (sig. <0.05).
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