Identifikasi Flavonoid dalam Fraksi Kloroform Daun Binahong (Anredera cordifolia (Tenore) Steen)
DOI:
https://doi.org/10.35473/ijpnp.v3i2.594Abstract
ABSTRAK
Fraksi kloroform daun binahong dari ekstrak etanoliknya telah dilaporkan dapat mempercepat penyembuhan luka pada hewan uji. Penelitian ini merupakan kelanjutan dari penelitian sebelumnya untuk mengetahui keberadaan senyawa flavonoid dalam fraksi kloroform daun binahong dengan metode kromatografi lapis tipis. Ekstrak etanol daun binahong diawalemakkan dengan n-heksan kemudian difraksinasi dengan kloroform menggunakan metode fraksinasi cair-padat. Selanjutnya fraksi dielusi dengan fase gerak n-heksan:etil asetat:metanol dengan perbandingan 6:3:1 dan fase diam silica gel 60F254. Deteksi semprot menggunakan pereaksi sitroborat yang sensitif terhadap senyawa flavonoid. Hasil KLT menunjukkan adanya 11 spot pada fraksi kloroform daun binahong yang di dalamnya tidak terdapat senyawa flavonoid.
Kata kunci : KLT, Fraksi Kloroform Daun Binahong, Flavonoid, Penyembuhan luka
Chloroform fraction of madeira vine leaf (FKDB) from the ethanolic extract (EEDB) was reported could promotes wound healing on gentle rat wistar animal testing. Quercetin flavonoid is the one of phytochemical that has wound healing pharmacology activity. Quercetin was found in the shoot part of madeira vine plants. The Leaf was located at the shoot part of plant that might contain flavonoid. This study was conduct to determine contain of quercetin in madeira vine’s leaf especially at chloroform fraction. EEDB made with maseration method. Madeira vine’s leaf powder soaked with ethanol 96% for 4 days and stirred for 3 hours then soaked again. Filtrate was taken after 24 hours and repeated until 4 days with solvent replacement. The extract was concentrated using rotary evaporator at50o C until concentrated. The solven evaporated using waterbath at the same temperature. Extract was defatted with n-hexane and fracionated with chloroform using liquid-solid method until obtain FKDB. Identification of phytochemical compounds in the chloroform fraction of madeira vine’s leaves by thin layer chromatography method. The fraction eluted in saturated chamber using mobile phase mixture of n-hexan:ethyl asetate:methanol with comparison 6:3:1 and activated silica gel 60F254 as stationary phase. Spray detection using citroborat reagent which is sensitive   to flavonoids. The result identificated using UV 254 and UV 366 showed that any 11 spot in the chloroform fraction of madeira vine’s leaves with no presense quercetin flavonoid there.
References
Astuti, S. M., Sakinah, M., Andayani, R., & Risch, A. (2011). Determination of Saponin Compound from Anredera cordifolia (Ten) Steenis Plant (Binahong) to Potential Treatment for Several Diseases. Journal of Agricultural Science, 3(4).
Bahramsoltani, R., Farzaei, M. H., & Rahimi, R. (2014). Medicinal plants and their natural components as future drugs for the treatment of burn wounds: An integrative review. In Archives of Dermatological Research 306(7), 601–617
Ekaviantiwi, T., Fachriyah, E., & Kusrini, D. (2013). Identifikasi asam fenolat dari ekstrak etanol daun binahong (Anredera cordifolia (Ten.) Stennis) dan uji aktivitas antioksidan. Chem Info, 1(1), 283–293.
Elfasyari, T. Y., Kintoko, K., & Nurkhasanah, N. (2018, December). Gambaran Penyembuhan Luka Tikus Diabetes Dengan Fraksi Etil Asetat Daun Binahong (Anredera cordifolia (Tenore) Steenis). In Talenta Conference Series: Tropical Medicine (TM) (Vol. 1, No. 3, pp. 158-161).
Kintoko, Karimatulhajj, H., Yuni Elfasyari, T., Arviana Ihsan, E., Adiyas Putra, T., Hariadi, P., Ariani, C., & Nurkhasanah. (2017). Effect of Diabetes Condition on Topical Treatment of Binahong Leaf Fraction in Wound Healing Process Pengaruh Kondisi Diabetes pada Pemberian Topikal Fraksi Daun Binahong dalam Proses Penyembuhan Luka. Traditional Medicine Journal, 22(2), 103–110.
Lordani, T., Lara, C. de, Ferreira, F. B. P., Monich, M. de S. T., Silva, C. M. da, Lordani, C. R. F., Bueno, F. G., Teixeira, J. J. V., & Lonardoni, M. V. C. (2018). Therapeutic effects of medicinal plants on cutaneous wound healing in humans: a systematic review. Hindawi, 2018, 1–12.
Mulia, K., Muhammad, F., & Krisanti, E. (2017). Extraction of vitexin from binahong (Anredera cordifolia (Ten.) Steenis) leaves using betaine-1,4 butanediol natural deep eutectic solvent. Aip.Scitation.Org, 1823, 20018.
Murdianto, A. (2013). Isolasi, Identifikasi Serta Uji Aktivitas Antibakteri Senyawa Golongan Triterpenoid Dari Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steen) Terhadap. Chem Info, 1(1).
Pandey, G., Verma, K. K. & Singh, M. (2014). Evaluation of phytochemical, antibacterial and free radical scavenging properties of Azadirachta indica (neem) leaves, Int. J. Pharm. Pharm. Sci , 6(2), 444–447.
Putra, A. A. G. R. Y., & Farm, S. (2020). Isolasi dan Karakterisasi Senyawa Flavonoid Potensial Antioksidan dari Daun Binahong (Anredera scandens (L.) Moq.). Jurnal Farmasi Udayana, 8(2).
Rahimi, R., Farzaei, M. H., Abbasabadi, Z., Mohammad, ;, Shams-Ardekani, R., & Abdollahi, ; Mohammad. (2014). A Comprehensive Review of Plants and Their Active Constituents With Wound Healing Activity in Traditional Iranian Medicine. WOUNDS, 26(7), 197–206.
Selawa, W., Runtuwene, M. R., & Citraningtyas, G. (2013). Kandungan flavonoid dan kapasitas antioksidan total ekstrak etanol daun binahong [Anredera cordifolia (Ten.) Steenis.]. Pharmacon, 2(1).
Süntar, I. P., Akkol, E. K., Yalçın, F. N., Koca, U., Keleş, H., & Yesilada, E. (2010). Wound healing potential of Sambucus ebulus L. leaves and isolation of an active component, quercetin 3-O-glucoside. Journal of ethnopharmacology, 129(1), 106-114.
Titis, M. B., Fachriyah, E., & Kusrini, D. (2013). Isolasi, Identifikasi dan Uji Aktifitas Senyawa Alkaloid Daun Binahong (Anredera cordifolia (Tenore) Steenis). Chem Info, 1(1), 196–201.
Yang RY, Lin S & Kuo G. (2008). Content and distribution of flavonoids among 91 edible plant species. Asia Pacific Journal of Clinical Nutrition, 17, 275-279.
